Alternative polyadenylation (APA) is an important RNA processing mechanism that regulates gene expression by generating diverse mRNA isoforms that may differ in stability, localisation and translation capacity. APA is often dysregulated in cancer, with some APA events shown to promote cancer cell growth and several studies reporting an association between APA dysregulation and poor patient prognosis. There is some evidence that key cellular nutrient sensing pathways may influence the APA landscape. Nutrient availability is often compromised within the tumour microenvironment and cancer cells rely on nutrient sensing pathways to restore metabolic homeostasis. This project aims to investigate the impact of altered nutrient availability on APA. Using Poly(A)-ClickSeq, we have demonstrated that cancer cells exhibit widespread alterations in APA in response to depletion of nutrients that are often restricted in the tumour microenvironment. We have observed changes common across all nutrient deprivation conditions, as well as nutrient-specific changes. Further, of the changes observed, we have identified a subset of gene-specific APA events associated with corresponding changes in mRNA expression. Overall, our results demonstrate an important link between nutrient availability, cellular metabolism and APA which requires further exploration. In future, we aim to interrogate the mechanism by which nutrient availability and nutrient sensing pathways regulate APA to identify key drivers of APA remodelling. Additionally, using a targeted approach, we aim to characterise the functional consequence of certain APA alterations on cancer progression and therapy resistance.